I used First Response 1 day after my missed period and it was :BFP. I went to the doctor and he did a test and it was :BFN. The results never came up on the docter's office test until like 6 or 7 days after my missed period. And remember with First Response a line is a line mine was faint I thought maybe I wasen't pregnant but here I am now 11 weeks pregnant. Good Luck girls